Study of diuretic activity of drugs using rats or mice

Aim: To study the effects of various drugs (diuretics) on the output of urine.

Principle: Diuretics are compound which increases the flow of urine. Normal urine output in rats is very small (1-2 ml/rat/day). Hence to get the measurable quantity the animals are first hydrated. The urine output is increased after the administration of diuretics like urea, hydroflumethiazide, and frusemide. An increase in the volume of urine is measured with the help of a measuring cylinder and compared with the normal urine output.


Rats, metabolic cages, graduated measuring cylinder.

Drugs and solutions: Normal saline(0.9%)

Urea (900 mg/kg;oral)

Hydroflumethiazide (1mg/kg;oral)



  1. albino rats (150-200 g) are fasted (deprived of food and water) overnight and saline(25ml/kg) is administered orally with the help of an oral feeding cannula.
  2. Those animals are divided into four groups containing three rats in each as follows: (i) Ist group- only normal saline (N.Saline) (ii) IInd group- saline + Urea (900 mg/kg; oral) (iii) IIIrd group-saline +hydroflumethiazide(1mg/kg; oral) (iv) IVth group-saline +frusemide(5mg/kg; oral)
  3. After the administration of drugs, animals are placed in four different metabolic cages.
  4. Urine is collected in a measuring cylinder.
  5. The time, when the first drop of urine is collected in a cylinder for each group is noted and the volume is recorded at intervals of 15 min for 3-4 hrs.
  6. The difference in the volume collected at different time intervals and total volume can be compared with various diuretics.



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